Violet Dye 5 Kg*
Violet Dye,” most commonly referred to as “Crystal Violet,” is primarily used as a stain to differentiate between Gram-positive and Gram-negative bacteria in a technique called Gram staining, where it acts as the primary stain, allowing researchers to identify different types of bacteria based on their cell wall structure; it can also be used in cell viability assays to quantify the number of living cells by binding to their cellular components like DNA and proteins.
Key points about Violet Dye (Crystal Violet) in a lab:
Gram Staining:
The primary application of Crystal Violet is in Gram staining, where it is used to initially stain bacteria on a microscope slide, allowing for differentiation between Gram-positive (purple) and Gram-negative (pink) bacteria based on their cell wall composition.
Cell Viability Assays:
Crystal Violet can be used to assess cell viability by staining adherent cells in a culture; dead cells detach, resulting in less dye retained, allowing for quantification of living cells.
